13C Urea Breath Test For Helicobacter Pylori Infection

13C Urea Breath Test For Helicobacter Pylori Infection

The 13C Urea Breath Test is the best approach to the diagnosis of H. pylori infection, with high sensitivity and specificity, and excellent performances.
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13C urea breath test for Helicobacter pylori infection

The urea breath test (UBT) is one of the most important non-invasive methods for detecting Helicobacter pylori infection. The test exploits the hydrolysis of orally administered urea by the enzyme urease, which H pylori produces in large quantities. Urea is hydrolysed to ammonia and carbon dioxide, which diffuses into the blood and is excreted by the lungs. Isotopically labelled CO2can be detected in breath using various methods.


Labelling urea with 13C is becoming increasingly popular because this non-radioactive isotope is innocuous and can be safely used in children and women of childbearing age. Breath samples can also be sent by post or courier to remote analysis centres. The test is easy to perform and can be repeated as often as required in the same patient. A meal must be given to increase the contact time between the tracer and the H pylori urease inside the stomach. The test has been simplified to the point that two breath samples collected before and 30 minutes after the ingestion of urea in a liquid form suffice to provide reliable diagnostic information. The cost of producing 13C-urea is high, but it may be possible to reduce the dosage further by administering it in capsule form.


These promising advances will certainly promote the wider use of the13C-UBT, which is especially useful for epidemiological studies in children and adults, for screening patients before endoscopy, and for assessing the efficacy of eradication regimens.


Many diagnostic methods have been developed over the past 15 years to detect Helicobacter pyloriinfection—some invasive (rapid urease test, histology, culture, and polymerase chain reaction) because they cannot be performed without endoscopy, and others non-invasive (serology, urea breath test (UBT) and, more recently, H pylori antigen determination on faeces). Of the latter, the UBT is being increasingly used both in pretreatment and post-treatment phases

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Richen 13C Urea Breath Test Products---Certified by European H Pylori Study Group

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